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 제품안내  분석 제작 서비스   유전자, 단백질 제작서비스

CRISPR/Cas9 Genome Editing Services

CRISPR/Cas9 유전자 편집을 위한 plasmid / sgRNA / ssDNA/ 제작 서비스를 제공합니다.

GenScript
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   1. GenCRISPR gRNA/Cas9 Plasmids

  • Pre-validated CRISPR plasmids (SpCas9/SpCas9 Nickase/SaCas9/Synergistic Activation Mediator(SAM))
  • Guide RNA design: gRNA sequence design tool 제공

    

Product

Vector

Selection

GenCRISPR™ Plasmid Collection

Lentiviral

Amp, Puro

eSpCas9 Plasmids

Lentiviral

Puro or GFP

SpCas9 Plasmids

Lentiviral/AAV

Amp
Amp, Puro
Amp, Neo
Amp, GFP

SpCas9 Nickase Plasmids

Lentiviral

Amp
Amp, Puro
Amp, GFP

SaCas9 Plasmids

AVV

Amp

SAM gRNA Plasmids

Lentiviral

Amp
Amp, Zeo

SAM dCas9-VP64 Plasmids

Lentiviral

Amp, Blast
Amp, GFP

SAM MS2-P65-HSF1

Lentiviral

Amp, GFP
Amp, Hygro

eSpCas9 and SpCas9 Broad

Lentiviral

Amp, Puro
Amp, GFP

SpCas9 Nickase

Plasmid

Amp
Amp, Puro
Amp, GFP

SaCas9

AAV

Amp

 

   2. Synthetic gRNAs & Cas9 nucleases

  • CRISPR/Cas9 ribonucleoprotein (RNP) complexes consists of Cas 9 proteins and synthetic single-guide RNA (sgRNA) duplex
  • DNA free gene editing
  • Less off-target effects
  • Ideal for in vivo gene editing

Product

Reagent

sgRNA

CRISPR/Cas9 sgRNA

crRNA & tracrRNA

CRISPR/Cas9 crRNA
CRISPR/Cas9 tracrRNA
Nuclease-Free Annealing Buffer, 5X

Cas9 Protein

GenCRISPR NLS-Cas9-NLS Nuclease (1 µg/µL)
Cas9 Nuclease Reaction Buffer, 10X

HPRT Control

HPRT Primer Mix (Human)
HPRT Positive Control crRNA (Human)

Other Products

Lipofection or Electroporation Materials


   3. Single-Stranded DNA synthesis

  • High efficient/Low off-target
  • Ideal for editing primary cell/stem cell/animal model
  • Minimum DNA base damage
  • Deliverables: Lyophilized form , Sequence verified via Sanger sequencing the final ssDNA product, Purity tested by gel electrophoresis

 

Length (Nucleotides)

Quantity

151-500

3/5/10/20/>20 ug

501-3000

3/5/10/20/>20 ug

3000-5000

inquiry

 

  4. Customized CRISPR Libraries

  • No sequence restriction
  • Top uniform distribution
  • NGS report upon request
  • 90,000 sgRNA per library



  5. Mammalian Cell Line Services

  • 풍부한 gRNA design 경험
  • Cell pool부터 single clone selection까지
  • 추가 서비스: WB, FACS, Promoter activity, Off-target analysis

  6. Microbial Genome Editing

  • Multigene editing: 3종 유전자를 동시에 knock-out 가능
  • Precision down to the base pair
  • Easy selection: selectable marker 필요 없음

 

Service

E. coli Knock-out

E. coli knock-in

Guide RNA Design

Our expert scientists will optimize your guide RNA sequence for maximized efficiency and minimal off-target effects using design algorithms created at the Broad Institute of MIT and Harvard.

Materials Needed from Clients

Host strain
Target gene name
Target gene sequence, if the whole genome sequence is unavailable

Multiplex Editing

Up to 3 targets

Single knock-in/replacement

Deliverables

Engineered strain in glycerol stock

Quality Control

Sequence Chromatogram
Quality Assurance Certificate
Quantitative RT-PCR Validation (Optional)
Western Blot Validation (Optional)

Delivery Time

Starting from 4 weeks