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 제품안내  분자생물학   PCR Amplification  Routine PCR

Taq DNA Polymerase

Escherichia coli로부터 정제된 94kDa의 내열성 Thermus aquaticus DNA polymerase로, native Taq DNA polymerase와 기능적으로 동등한 성질을 갖고 있습니다.

KOMABIOTECH
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    특징
  • 고순도로 정제된 recombinant Taq polymerase 입니다.
  • Taq polymerase뿐만 아니라 reaction buffer, dNTP mixture, magic buffer, nuclease free water, loading buffer가 함께 제공되어 사용이 편리합니다.
  • MgCl2 필요여부에 따라 reaction buffer를 선택적으로 구매할수 있습니다.
  • Magic Buffer 사용으로 증폭하기 어려운 구조의 target DNA도 증폭이 수월합니다.
  • 일반적인 PCR application에 적합합니다.
  • 증폭길이
    • lambda DNA: ~ 6kb까지 (최대 12kb)
    • human genomic DNA: ~2kb까지 (최대 4Kb)
    구성
    Description Component Cat. No
    Taq DNA Polymerase Kit I Taq DNA Polymerase I K0561001/K0561002/K0561003
    10X Taq PCR Buffer I (with MgCl2) K0561011
    dNTP Mix (2.5mM each) K1756011
    10X Loading Buffer K0561051
    Nuclease Free Water K0561061
    4X Magic Buffer (for high GC content) K0561031
    Taq DNA Polymerase Kit II Taq DNA Polymerase II K0561004/K0561005/K0561006
    10X Taq PCR Buffer II (MgCl2 free) K0561012
    MgCl2 Stock solution K0561041
    dNTP Mix (2.5mM each) K1756011
    10X Loading Buffer K0561051
    Nuclease Free Water K0561061
    4X Magic Buffer K0561031
    적용
    Figure 1. PCR for human beta-globin gene (A) and lambda DNA (B) were performed using a serial dilution of Taq DNA Polymerase.
    Figure 2. The Magic buffer is very useful to amplify DNA of high GC contents or of complicated structure. Human retinoblastoma gene (A), human apolipoprotein E gene (B) and human Htra serine protease1 gene (C) were amplified by addition of Magic buffer to Taq DNA Polymerase.
주문정보(Ordering Information)
CATNUM PRODUCT SIZE
K0561031Magic Buffer, 4X 1ml
K0561041MgCl2 Stock Solution1.5ml
K0561061Nuclease Free Water1ml
K0560050Taq DNA Polymerase I5000U
K0560010Taq DNA Polymerase I1000U
K0560350Taq DNA Polymerase II (MgCl2 Free Reaction Buffer)5000U
K0560310Taq DNA Polymerase II (MgCl2 Free Reaction Buffer)1000U
K0561003Taq DNA Polymerase Kit I1000U
K0561002Taq DNA Polymerase Kit I500U
K0561001Taq DNA Polymerase Kit I250U
K0561006Taq DNA Polymerase Kit II (MgCl2 Free Reaction Buffer)1000U
K0561005Taq DNA Polymerase Kit II (MgCl2 Free Reaction Buffer)500U
K0561004Taq DNA Polymerase Kit II (MgCl2 Free Reaction Buffer)250U
K0561011Taq PCR Buffer I, 10X (w/ MgCl2)1.5ml
K0561012Taq PCR Buffer II, 10X (w/o MgCl2)1.5ml