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 ǰȳ  ü,׿,ŶƮ   FAQ

[FAQ] Oxidative Stress Assay Kit

ǰ ϱ
μϱ



1. Oxidative stress  ϳ?
Oxidative stress Ϲ 3 ˴ϴ.
ROS(Reaction Oxygen Species) θ Ȯϴ anti-oxidant θ ̿ , ׸ ȭ ջ ܹ, , DNA, RNA ̿ ֽϴ. Ϲ ROS Ҿϱ ܹ, , DNA, RNA ϴ ַ ̿˴ϴ. Ư Protein Carbonyl, 8-OHdG, TBARS ϴ ˴ϴ. 
2. Species Oxidative Stress Assay Kit ٸ ؾ ϳ?
Species-independent ǰ м մϴ. Biomolecule ƴ Oxidative stress Damage marker մϴ.  
3. Marker /Ұ Ÿ ֳ?
  ǥ Marker ִ sample type Ȯغ.
Marker or Type of Damage Sample Type
Cells Tissues Blood Urine Other
DNA / RNA Damage
8-hydroxyguanosine (8-OHG) X X X X Cerebrospinal fluid
8-hydroxydeoxyguanosine (8-OHdG) X X X X
Abasic (AP) sites X X
BPDE DNA Adduct X X
Double-strand DNA breaks X
Comet Assay (general DNA damage) X
UV DNA Damage (CPD, 6-4PP) X
Lipid Peroxidation
4-Hydroxynonenal (4-HNE) X X X
8-iso-Prostaglandin F2alpha (8-isoprostane) X X X X
Malondialdehyde (MDA) X X X X
TBARS X X X X
Protein Oxidation / Nitration
Protein Carbonyl Content (PCC) X X X
3-Nitrotyrosine X X X
Advanced Glycation End Products (AGE) X X X
Advanced Oxidation Protein Products (AOPP) X X X
BPDE Protein Adduct X X X
Kidney Injury Molecule-1 (KIM-1) X
Reactive Oxygen Species
Intracellular ROS X
In Vitro ROS X X X X
Hydrogen Peroxide X X X X
Nitric Oxide X
Antioxidants
Catalase X X X
Glutathione X X X X
Superoxide Dismutase X X X
Oxygen Radical Antioxidant Capacity (ORAC) X X X X Food samples
Hydroxyl Radical Antioxidant Capacity (HORAC) X X X X Food samples
Total Antioxidant Capacity (TAC) X X X X Food samples
Cellular Antioxidant Assay Food samples, Antioxidants
4. 8-OHdG DNA Damage ELISA(Cat #. STA-320) , Sample DNA Extraction ٷ Assay ؾ ϳ?
8-OHdG DNA ſ ̸, -80°C 1⵿ մϴ. 
5. OxiSelect™ 8-OHdG ELISA Kit(Cat #. STA-320) ʿ ּ DNA Դϱ?
8-OHdG Ȯϰ detectionϱ ؼ well ּ 2 ug DNA ʿմϴ. 
6. Human 8-isoprostane (8-iso-Prostaglandin F2α)  ˴ϱ?
Human plasma 40-100 pg/mL, Urine 500-2000 pg/mL 󵵷 8-isoprostane մϴ. 
7. Serum Protein oxidation Ȯ , PBS Ȥ BSA ̿Ͽ Serum dilution ʿմϱ?
м BCA Ȥ Bradford assay Ͽ Protein 󵵸 Ȯ , 10 ug/mL 󵵰 ǵ PBS ؾ մϴ. 
6. Superoxide Dismutase Activity Assay Kit ̿Ͽ Superoxide dismutase ֳ?
SOD enzymes cytosolic Cu/Zn-SOD, mitochondrial Mn-SOD, extracellular Ec-SOD з˴ϴ. OxiSelect™ Superoxide Dismutase Activity Assay Kit(Cat #. STA-340) SOD ֽϴ. 
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