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 제품안내  분자생물학   COVID-19 Research

[COVID-19] Primer/probe/plasmid/qPCR kit

신종코로나바이러스 (COVID-19) 검출을 위한 detection kit 및 primer/probe/positive control plasmid를 공급합니다.

GenScript
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 ▶ SARS-CoV-2 qRT-PCR Detection Assay

  • 높은 specificity를 갖는 FAM probe를 이용한 TaqMan qRT-PCR 분석 킷트 입니다.
  • GenBank Sequence NC_045512.2에 근거한 4가지 gene을 타겟팅 하였습니다. (ORF1ab, RdRP, N, E)
  • 이 4가지 gene을 포함한 positive sample을 성공적으로 검출할 수 있습니다.
  • Specimen type：Bronchoalveolar fluid(BALF), Oral Swab
  • MasterMix
  • Forward Primer
  • Reverse Primer
  • Probe
  • RNase-free water
  • Positive control plasmid

  • 1-step/1-plex assay : ORF1ab, RdRP, N, E gene을 각각 검출할 수 있는 개별 킷트
  • 1-step/2-plex assay : ORF1ab+N gene 혹은 RdRP+E gene을 동시에 검출할 수 있는 다중 킷트
　  1-step, 1-plex assay  Cat. No
　  ORF1ab gene  문의
　  N gene  문의
　  RdRP gene  문의
　  E gene  문의
　 　 　
　  1-step, 2-plex assay  Cat. No
　  ORF1ab + N gene  문의
　  RdRP + E gene  문의

 

 ▶ Synthesized primer/probes 

  • WHO(RdRP & E)와 CDC(N & ORF1ab)에 의해 발표된 gene에 대한 primer/probe 입니다.
  • HPLC purified
  • NTC No Ct value within 40 cycles

 Gene Name  oligo Name Length (nt) 5'mod 3'mod
 RdRP gene  RdRP_SARSr-F2  22 　 　
 RdRP_SARSr-R1  26 　 　
 RdRP_SARSr-P2  25 FAM BHQ-1
 RdRP_SARSr-P1  26 FAM BHQ-1
 E gene  E_Sarbeco_F1  26 　 　
 E_Sarbeco_R2  22 　 　
 E_Sarbeco_P1 26 FAM BHQ-1
 N gene  N gene-F 22 　 　
 N gene-R 22 　 　
 N gene-P 20 FAM TARMA
 ORF1ab gene  ORF1ab-F 21 　 　
 ORF1ab-R 19 　 　
 ORF1ab-P 28 FAM BHQ-1

 

 ▶ Positive Control Plasmid
  • RT-PCR로 2019-nCoV 검출시, positive control로 이용될 수 있는 plasmid 입니다. 
  • 2019-nCoV의 surface glycoprotein과 nucleocapsid phosphoprotein을 encoding하는 gene 및 Homo sapiens angiotensin I converting enzyme 2 (ACE2) gene을 포함하는 plasmid를 제공합니다.

 Cat.No. Description Detail
 MC_0101087 2019-nCov_pcDNA3.1(+)-P2A-eGFP The plasmid is used for 2019-nCoV(SARS-CoV-2) surface glycoprotein expression (Codon Optimized for Mouse expression system).
The surface glycoprotein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
 MC_0101088 SARS_pcDNA3.1(+)-P2A-eGFP The plasmid is used for SARS surface glycoprotein expression (Codon Optimized for Mouse expression system).
The surface glycoprotein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
 MC_0101089 2019-nCov-Linker_pcDNA3.1(+)-C-eGFP The plasmid is used for 2019-nCoV(SARS-CoV-2) surface glycoprotein expression (Codon Optimized for Mouse expression system).
The surface glycoprotein is integrated with eGFP protein through linker, which can be used for flow cytometry antibody screening.
 MC_0101090 SARS-Linker_pcDNA3.1(+)-C-eGFP The plasmid is used for SARS surface glycoprotein expression (Codon Optimized for Mouse expression system).
The surface glycoprotein is integrated with eGFP protein through linker, which can be used for flow cytometry antibody screening.
 MC_0101129 pcDNA3.1(+)-P2A-eGFP-ORF10 new  The plasmid is used for SARS-CoV-2 ORF10 protein expression.
The ORF10 protein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
 MC_0101130 pcDNA3.1(+)-P2A-eGFP-ORF8 new  The plasmid is used for SARS-CoV-2 ORF8 protein expression.
The ORF8 protein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
 MC_0101131 pcDNA3.1(+)-P2A-eGFP-ORF6 new  The plasmid is used for SARS-CoV-2 ORF6 protein expression.
The ORF6 protein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
 MC_0101132 pcDNA3.1(+)-P2A-eGFP-ORF3a new  The plasmid is used for SARS-CoV-2 ORF3a protein expression.
The ORF3a protein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
 MC_0101133 pcDNA3.1(+)-P2A-eGFP-ORF7a new  The plasmid is used for SARS-CoV-2 ORF7a protein expression.
The ORF7a protein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
 MC_0101134 pcDNA3.1(+)-P2A-eGFP-ORF7b new  The plasmid is used for SARS-CoV-2 ORF7b protein expression.
The ORF7b protein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
 MC_0101135 pcDNA3.1(+)-P2A-eGFP-E Protein new  The plasmid is used for SARS-CoV-2 envelope protein protein expression.
The envelope protein n is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
 MC_0101136 pET-28a(+)-M Protein new  The plasmid is used for SARS-CoV-2 membrane glycoprotein expression.
 MC_0101137 pcDNA3.1(+)-N-eGFP-N Protein new  The plasmid is used for SARS-CoV-2 nucleocapsidphos phoprotein expression.
 MC_0101076 pUC57-2019-nCoV-PC:RdRP  This plasmid contains part of 2019-nCoV(SARS-CoV-2) RdRP gene and can be used as positive control for the detection of 2019-nCoV(SARS-CoV-2) by qRT-PCR
 MC_0101077 pUC57-2019-nCoV-PC:N  This plasmid contains part of 2019-nCoV(SARS-CoV-2) N gene and can be used as positive control for the detection of 2019-nCoV(SARS-CoV-2) by qRT-PCR
 MC_0101078 pUC57-2019-nCoV-PC:E  This plasmid contains part of 2019-nCoV(SARS-CoV-2) E gene and can be used as positive control for the detection of 2019-nCoV(SARS-CoV-2) by qRT-PCR
 MC_0101079 pUC57-2019-nCoV-PC:ORF1ab  This plasmid contains part of 2019-nCoV(SARS-CoV-2) ORF1ab and can be used as positive control for the detection of 2019-nCoV(SARS-CoV-2) by qRT-PCR
 MC_0101080 pUC57-2019-nCoV-S(Original)  This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) surface glycoprotein (original sequence)
 MC_0101081 pUC57-2019-nCoV-S(Human)  This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) surface glycoprotein (Codon optimized for Human expression system)
 MC_0101082 pUC57-2019-nCoV-S(E. coli)  This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) surface glycoprotein (Codon optimized for E. Coli expression system)
 MC_0101083 pUC57-2019-nCoV-S(CHO)  This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) surface glycoprotein (Codon optimized for CHO expression system)
 MC_0101084 pUC57-2019-nCoV-S(Insect)  This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) surface glycoprotein (Codon optimized for Insect expression system)
 MC_0101085 pUC57-2019-nCoV-N  This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) nucleocapsid phosphoprotein (original sequence)
 MC_0101086 pcDNA3.1+/C-(K)DYK-ACE2 (NM_021804.2, OHu20260)  Homo sapiens angiotensin I converting enzyme 2 (ACE2), the receptor for 2019-nCoV(SARS-CoV-2).
 

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