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 Á¦Ç°¾È³»  ºÐÀÚ»ý¹°ÇР  COVID-19 Research

[COVID-19] GenScript - Primer/probe/plasmid/qPCR kit

½ÅÁ¾Äڷγª¹ÙÀÌ·¯½º (COVID-19) °ËÃâÀ» À§ÇÑ detection kit ¹× primer/probe/positive control plasmid¸¦ °ø±ÞÇÕ´Ï´Ù.

GenScript
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 ¢º SARS-CoV-2 qRT-PCR Detection Assay

  • ³ôÀº specificity¸¦ °®´Â FAM probe¸¦ ÀÌ¿ëÇÑ TaqMan qRT-PCR ºÐ¼® ŶƮ ÀÔ´Ï´Ù.
  • GenBank Sequence NC_045512.2¿¡ ±Ù°ÅÇÑ 4°¡Áö geneÀ» Ÿ°ÙÆÃ ÇÏ¿´½À´Ï´Ù. (ORF1ab, RdRP, N, E)
  • ÀÌ 4°¡Áö geneÀ» Æ÷ÇÔÇÑ positive sampleÀ» ¼º°øÀûÀ¸·Î °ËÃâÇÒ ¼ö ÀÖ½À´Ï´Ù.
  • Specimen type£ºBronchoalveolar fluid(BALF), Oral Swab
  • MasterMix
  • Forward Primer
  • Reverse Primer
  • Probe
  • RNase-free water
  • Positive control plasmid

  • 1-step/1-plex assay : ORF1ab, RdRP, N, E geneÀ» °¢°¢ °ËÃâÇÒ ¼ö Àִ °³º° ŶƮ
  • 1-step/2-plex assay : ORF1ab+N gene ȤÀº RdRP+E geneÀ» µ¿½Ã¿¡ °ËÃâÇÒ ¼ö ÀÖ´Â ´ÙÁß Å¶Æ®
¡¡  1-step, 1-plex assay  Cat. No
¡¡  ORF1ab gene  ¹®ÀÇ
¡¡  N gene  ¹®ÀÇ
¡¡  RdRP gene  ¹®ÀÇ
¡¡  E gene  ¹®ÀÇ
¡¡ ¡¡ ¡¡
¡¡  1-step, 2-plex assay  Cat. No
¡¡  ORF1ab + N gene  ¹®ÀÇ
¡¡  RdRP + E gene  ¹®ÀÇ

 

 ¢º Synthesized primer/probes 

  • WHO(RdRP & E)¿Í CDC(N & ORF1ab)¿¡ ÀÇÇØ ¹ßÇ¥µÈ gene¿¡ ´ëÇÑ primer/probe ÀÔ´Ï´Ù.
  • HPLC purified
  • NTC No Ct value within 40 cycles

 Gene Name  oligo Name Length (nt) 5'mod 3'mod
 RdRP gene  RdRP_SARSr-F2  22 ¡¡ ¡¡
 RdRP_SARSr-R1  26 ¡¡ ¡¡
 RdRP_SARSr-P2  25 FAM BHQ-1
 RdRP_SARSr-P1  26 FAM BHQ-1
 E gene  E_Sarbeco_F1  26 ¡¡ ¡¡
 E_Sarbeco_R2  22 ¡¡ ¡¡
 E_Sarbeco_P1 26 FAM BHQ-1
 N gene  N gene-F 22 ¡¡ ¡¡
 N gene-R 22 ¡¡ ¡¡
 N gene-P 20 FAM TARMA
 ORF1ab gene  ORF1ab-F 21 ¡¡ ¡¡
 ORF1ab-R 19 ¡¡ ¡¡
 ORF1ab-P 28 FAM BHQ-1

 

 ¢º Positive Control Plasmid
  • RT-PCR·Î 2019-nCoV °ËÃâ½Ã, positive control·Î ÀÌ¿ëµÉ ¼ö ÀÖ´Â plasmid ÀÔ´Ï´Ù. 
  • 2019-nCoVÀÇ surface glycoprotein°ú nucleocapsid phosphoproteinÀ» encodingÇÏ´Â gene ¹× Homo sapiens angiotensin I converting enzyme 2 (ACE2) geneÀ» Æ÷ÇÔÇϴ plasmid¸¦ Á¦°øÇÕ´Ï´Ù.

 Cat.No. Description Detail
 MC_0101087 2019-nCov_pcDNA3.1(+)-P2A-eGFP The plasmid is used for 2019-nCoV(SARS-CoV-2) surface glycoprotein expression (Codon Optimized for Mouse expression system).
The surface glycoprotein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
 MC_0101088 SARS_pcDNA3.1(+)-P2A-eGFP The plasmid is used for SARS surface glycoprotein expression (Codon Optimized for Mouse expression system).
The surface glycoprotein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
 MC_0101089 2019-nCov-Linker_pcDNA3.1(+)-C-eGFP The plasmid is used for 2019-nCoV(SARS-CoV-2) surface glycoprotein expression (Codon Optimized for Mouse expression system).
The surface glycoprotein is integrated with eGFP protein through linker, which can be used for flow cytometry antibody screening.
 MC_0101090 SARS-Linker_pcDNA3.1(+)-C-eGFP The plasmid is used for SARS surface glycoprotein expression (Codon Optimized for Mouse expression system).
The surface glycoprotein is integrated with eGFP protein through linker, which can be used for flow cytometry antibody screening.
 MC_0101129 pcDNA3.1(+)-P2A-eGFP-ORF10 new  The plasmid is used for SARS-CoV-2 ORF10 protein expression.
The ORF10 protein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
 MC_0101130 pcDNA3.1(+)-P2A-eGFP-ORF8 new  The plasmid is used for SARS-CoV-2 ORF8 protein expression.
The ORF8 protein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
 MC_0101131 pcDNA3.1(+)-P2A-eGFP-ORF6 new  The plasmid is used for SARS-CoV-2 ORF6 protein expression.
The ORF6 protein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
 MC_0101132 pcDNA3.1(+)-P2A-eGFP-ORF3a new  The plasmid is used for SARS-CoV-2 ORF3a protein expression.
The ORF3a protein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
 MC_0101133 pcDNA3.1(+)-P2A-eGFP-ORF7a new  The plasmid is used for SARS-CoV-2 ORF7a protein expression.
The ORF7a protein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
 MC_0101134 pcDNA3.1(+)-P2A-eGFP-ORF7b new  The plasmid is used for SARS-CoV-2 ORF7b protein expression.
The ORF7b protein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
 MC_0101135 pcDNA3.1(+)-P2A-eGFP-E Protein new  The plasmid is used for SARS-CoV-2 envelope protein protein expression.
The envelope protein n is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
 MC_0101136 pET-28a(+)-M Protein new  The plasmid is used for SARS-CoV-2 membrane glycoprotein expression.
 MC_0101137 pcDNA3.1(+)-N-eGFP-N Protein new  The plasmid is used for SARS-CoV-2 nucleocapsidphos phoprotein expression.
 MC_0101076 pUC57-2019-nCoV-PC:RdRP  This plasmid contains part of 2019-nCoV(SARS-CoV-2) RdRP gene and can be used as positive control for the detection of 2019-nCoV(SARS-CoV-2) by qRT-PCR
 MC_0101077 pUC57-2019-nCoV-PC:N  This plasmid contains part of 2019-nCoV(SARS-CoV-2) N gene and can be used as positive control for the detection of 2019-nCoV(SARS-CoV-2) by qRT-PCR
 MC_0101078 pUC57-2019-nCoV-PC:E  This plasmid contains part of 2019-nCoV(SARS-CoV-2) E gene and can be used as positive control for the detection of 2019-nCoV(SARS-CoV-2) by qRT-PCR
 MC_0101079 pUC57-2019-nCoV-PC:ORF1ab  This plasmid contains part of 2019-nCoV(SARS-CoV-2) ORF1ab and can be used as positive control for the detection of 2019-nCoV(SARS-CoV-2) by qRT-PCR
 MC_0101080 pUC57-2019-nCoV-S(Original)  This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) surface glycoprotein (original sequence)
 MC_0101081 pUC57-2019-nCoV-S(Human)  This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) surface glycoprotein (Codon optimized for Human expression system)
 MC_0101082 pUC57-2019-nCoV-S(E. coli)  This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) surface glycoprotein (Codon optimized for E. Coli expression system)
 MC_0101083 pUC57-2019-nCoV-S(CHO)  This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) surface glycoprotein (Codon optimized for CHO expression system)
 MC_0101084 pUC57-2019-nCoV-S(Insect)  This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) surface glycoprotein (Codon optimized for Insect expression system)
 MC_0101085 pUC57-2019-nCoV-N  This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) nucleocapsid phosphoprotein (original sequence)
 MC_0101086 pcDNA3.1+/C-(K)DYK-ACE2 (NM_021804.2, OHu20260)  Homo sapiens angiotensin I converting enzyme 2 (ACE2), the receptor for 2019-nCoV(SARS-CoV-2).
 

 ¢º Custom Production Service for Vaccine development
ÁÖ¹®Á¤º¸(Ordering Information)
CATNUM PRODUCT SIZE
MC_01010892019-nCov-Linker_pcDNA3.1(+)-C-eGFP4ug
MC_01010872019-nCov_pcDNA3.1(+)-P2A-eGFP4ug
Primer-E gene_FE_Sarbeco_F1 (26mer)5/10/50/100/200 nmol
Probe-E_Sarbeco_P1E_Sarbeco_P1(26mer), 5'FAM/3'BHQ-15/10/50/100 nmol
Primer-E gene_RE_Sarbeco_R2 (22mer)5/10/50/100/200 nmol
Primer-N gene-FN gene-F (22mer)5/10/50/100/200 nmol
Probe-N gene-PN gene-P (20mer), 5'FAM/3'TARMA5/10/50/100 nmol
Primer-N gene-RN gene-R (22mer)5/10/50/100/200 nmol
Primer-ORF1ab-FORF1ab-F (21mer)5/10/50/100/200 nmol
Probe-ORF1ab-PORF1ab-P (28mer), 5'FAM/3'BHQ-15/10/50/100 nmol
Primer-ORF1ab-RORF1ab-R (19mer)5/10/50/100/200 nmol
MC_0101137pcDNA3.1(+)-N-eGFP-N Protein 4ug
MC_0101135pcDNA3.1(+)-P2A-eGFP-E Protein 4ug
MC_0101129pcDNA3.1(+)-P2A-eGFP-ORF10 4ug
MC_0101132pcDNA3.1(+)-P2A-eGFP-ORF3a 4ug
MC_0101131pcDNA3.1(+)-P2A-eGFP-ORF6 4ug
MC_0101133pcDNA3.1(+)-P2A-eGFP-ORF7a 4ug
MC_0101134pcDNA3.1(+)-P2A-eGFP-ORF7b 4ug
MC_0101130pcDNA3.1(+)-P2A-eGFP-ORF84ug
MC_0101086pcDNA3.1+/C-(K)DYK-ACE2 (NM_021804.2, OHu20260) 4ug
MC_0101136pET-28a(+)-M Protein 4ug
MC_0101085pUC57-2019-nCoV-N 4ug
MC_0101078pUC57-2019-nCoV-PC:E 4ug
MC_0101077pUC57-2019-nCoV-PC:N 4ug
MC_0101079pUC57-2019-nCoV-PC:ORF1ab 4ug
MC_0101076pUC57-2019-nCoV-PC:RdRP 4ug
MC_0101083pUC57-2019-nCoV-S(CHO) 4ug
MC_0101082pUC57-2019-nCoV-S(E. coli) 4ug
MC_0101081pUC57-2019-nCoV-S(Human) 4ug
MC_0101084pUC57-2019-nCoV-S(Insect) 4ug
MC_0101080pUC57-2019-nCoV-S(Original) 4ug
Primer-RdRP-FRdRP_SARSr-F2 (22mer)5/10/50/100/200 nmol
Probe-RdRP_SARSr-P1RdRP_SARSr-P1 (26mer), 5'FAM/3'BHQ-15/10/50/100 nmol
Probe-RdRP_SARSr-P2RdRP_SARSr-P2 (25mer), 5'FAM/3'BHQ-15/10/50/100 nmol
Primer-RdRP-RRdRP_SARSr-R1 (26mer)5/10/50/100/200 nmol
MC_0101090SARS-Linker_pcDNA3.1(+)-C-eGFP4ug
MC_0101088SARS_pcDNA3.1(+)-P2A-eGFP4ug