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 제품안내  분자생물학   Protein Research  Protein concentration & Extraction

Optima PRO Screens & De-salting

Optima PRO Screen은 다양한 종류의 단백질 샘플을 한 번에 정제 하기 위해 이용됩니다. 최대 96개의 서로 다른 샘플을 이미 resin이 coating된 plate에서 한 번에 정제 할 수 있습니다. (resin은 샘플에 맞게 선택할 수 있습니다.)

Protein Ark
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특징
  • Filtration과 purification을 한번에 진행할 수 있는 96 well screen plate 입니다.
  • 각 well에 resin이 처리되어 한번에 다량의 단백질을 정제할 수 있습니다.
  • Secreted protein은 전처리 없이 바로 cell culture 용액을 로딩합니다.
  • 막힐 염려가 없으므로 원심분리나 필터링등의 전처리가 필요없습니다.
  • 분리하고자 하는 단백질에 따라 Resin을 선택할 수 있습니다.

  Optima Pro Empty Plate Optima PRO-D Ni Advance plate Optima PRO-D Protein A plate Optima PRO-D Protein G plate Optima PRO-D Q-IEX plate Optima PRO-D S-IEX plate
Resin Resin dependent Fastback Ni Advance Fastback Protein A Fastback Protein G Fastback Q-IEX Fastback S-IEX
Specificity Resin dependent His - tag IgG, Fc resion IgG, Fc resion Negatively charged proteins Positively charged proteins
Matrix Resin dependent 6% cross-linked Agarose Sepharose Sepharose 6% cross-linked Agarose 6% cross-linked Agarose
Coupled ligand Resin dependent Ni chelating ligand 3.5 mg
Protein A /ml
2 mg
Protein G /ml
Quaternary ammonium Sulphonic acid propyl
Binding capacity Resin dependent 80 mg/ml 30 mg/ml 20 mg/ml 50 - 70 mg/ml 50 - 70 mg/ml
Bead size Resin dependent 90um 60 - 165 um 45 - 165 um 90 - 100 um 90 - 100 um
DTT stability (24 hours) Resin dependent 20mM - - - -
EDTA stability (24 hours) Resin dependent 20mM - - - -
Buffer compatibility Common aqueous buffers from pH 2-12 Common aqueous buffers from pH 4-9 Common aqueous buffers from pH 2.5-10 Common aqueous buffers from pH 2-9 Common aqueous buffers from pH 2-12 Common aqueous buffers from pH 2-12
Resin volume per well 50ul - 100ul 50ul
Diatomaceous Earth No Yes
Max recommended sample volume 1ml
plastic material Polypropylene
Membrane material PTFE
Recommended vacuum pressure -0.6 bar (max. vacuum -0.8 bar)
Recommended centrifuge speed 2,000 - 3,000 x g
Recommended shaker speed (2mm orbit) 800 rpm
Maximum shaker speed (2mm orbit) 1,200 rpm
Storage temperature Ambient 2 - 8 ℃






Sample type Optima PRO-D
(pre-packed with resin and Diatomaceous Earth)
Intact Mammalian cells (HEK/CHO) Unclarified sample
(Up to 15 x 10^6 cells/ml)
Intact Insect cells (Sf9/Hi5) Unclarified sample
(Up to 15 x 10^6 cells/ml)
Bacterial cell lysate (E.coil) Pre-clarification of sample
(5,000 x g for 15 mins in 2ml deep well block)
Mammalian cell lysate (HEK/CHO) Pre-clarification of sample
(5,000 x g for 15 mins in 2ml deep well block)
Insect cell lysate (Sf9/Hi5) Pre-clarification of sample
(5,000 x g for 15 mins in 2ml deep well block)



  • 다량의 샘플을 한번에 쉽게 탈염하거나 투석하는데 이용하는 plate 입니다.
  • De-salting에는 8분, Buffer exchange에는 12분이 소요됩니다.








주문정보(Ordering Information)
CATNUM PRODUCT SIZE
PAL-HT-S-1D1 Optima PRO Screen, 1 Pressure sealing plate, 2 wash/eluate collection plates (2 ml), 2 eluate collection plates (0.35 ml), 2 blotting membranes, 1 sealing matpk
PAL-HT-S-11 Optima PRO Screen, 1 Pressure sealing plate, 2 wash/eluate collection plates (2 ml), 2 eluate collection plates (0.35 ml), 2 blotting membranes, 1 sealing matpk
PAL-HT-Q-1D1 Optima PRO Screen, 1 Pressure sealing plate, 2 wash/eluate collection plates (2 ml), 2 eluate collection plates (0.35 ml), 2 blotting membranes, 1 sealing matpk
PAL-HT-Q-11 Optima PRO Screen, 1 Pressure sealing plate, 2 wash/eluate collection plates (2 ml), 2 eluate collection plates (0.35 ml), 2 blotting membranes, 1 sealing matpk
PAL-HT-PG-1D1 Optima PRO Screen, 1 Pressure sealing plate, 2 wash/eluate collection plates (2 ml), 2 eluate collection plates (0.35 ml), 2 blotting membranes, 1 sealing matpk
PAL-HT-PA-1D1 Optima PRO Screen, 1 Pressure sealing plate, 2 wash/eluate collection plates (2 ml), 2 eluate collection plates (0.35 ml), 2 blotting membranes, 1 sealing matpk
PAL-HT-PA-11 Optima PRO Screen, 1 Pressure sealing plate, 2 wash/eluate collection plates (2 ml), 2 eluate collection plates (0.35 ml), 2 blotting membranes, 1 sealing matpk
PAL-HT-NiADV-1D1 Optima PRO Screen, 1 Pressure sealing plate, 2 wash/eluate collection plates (2 ml), 2 eluate collection plates (0.35 ml), 2 blotting membranes, 1 sealing matpk
PAL-HT-NiADV-11 Optima PRO Screen, 1 Pressure sealing plate, 2 wash/eluate collection plates (2 ml), 2 eluate collection plates (0.35 ml), 2 blotting membranes, 1 sealing matpk
PAL-HT-HEP-1D1 Optima PRO Screen, 1 Pressure sealing plate, 2 wash/eluate collection plates (2 ml), 2 eluate collection plates (0.35 ml), 2 blotting membranes, 1 sealing matpk
PAL-HT-EMP-11 Optima PRO Screen, 1 Pressure sealing plate, 2 wash/eluate collection plates (2 ml), 2 eluate collection plates (0.35 ml), 2 blotting membranes, 1 sealing matpk
PRO96-10x10 De-salting platespk
PRO96-1010 Optima PRO De-salting plates, 10 collection plates (0.35 ml), 10 waste plates (0.5 ml) 10 plate lidspk
PAL-HT-PG-10D10 Optima PRO Screens, 2 Pressure sealing plates, 20 blotting membranes, 10 sealing matspk
PAL-HT-S-10D10 Optima PRO Screens, 2 Pressure sealing plates, 20 blotting membranes, 10 sealing matspk
PAL-HT-S-1010 Optima PRO Screens, 2 Pressure sealing plates, 20 blotting membranes, 10 sealing matspk
PAL-HT-Q-10D10 Optima PRO Screens, 2 Pressure sealing plates, 20 blotting membranes, 10 sealing matspk
PAL-HT-Q-1010 Optima PRO Screens, 2 Pressure sealing plates, 20 blotting membranes, 10 sealing matspk
PAL-HT-PA-10D10 Optima PRO Screens, 2 Pressure sealing plates, 20 blotting membranes, 10 sealing matspk
PAL-HT-PA-1010 Optima PRO Screens, 2 Pressure sealing plates, 20 blotting membranes, 10 sealing matspk
PAL-HT-NiADV-10D10 Optima PRO Screens, 2 Pressure sealing plates, 20 blotting membranes, 10 sealing matspk
PAL-HT-NiADV-1010 Optima PRO Screens, 2 Pressure sealing plates, 20 blotting membranes, 10 sealing matspk
PAL-HT-HEP-10D10 Optima PRO Screens, 2 Pressure sealing plates, 20 blotting membranes, 10 sealing matspk
PAL-HT-EMP-1010 Optima PRO Screens, 2 Pressure sealing plates, 20 blotting membranes, 10 sealing matspk
PRO96-22 Optima PRO De-salting plates, 2 collection plates (0.35 ml), 2 waste plates (0.5 ml) 2 plate lidspk
PAL-HT-S-2D2 Optima PRO Screen, 2 Pressure sealing plate, 4 wash/eluate collection plates (2 ml), 4 eluate collection plates (0.35 ml), 4 blotting membranes, 2 sealing matspk
PAL-HT-S-22 Optima PRO Screen, 2 Pressure sealing plate, 4 wash/eluate collection plates (2 ml), 4 eluate collection plates (0.35 ml), 4 blotting membranes, 2 sealing matspk
PAL-HT-Q-2D2 Optima PRO Screen, 2 Pressure sealing plate, 4 wash/eluate collection plates (2 ml), 4 eluate collection plates (0.35 ml), 4 blotting membranes, 2 sealing matspk
PAL-HT-Q-22 Optima PRO Screen, 2 Pressure sealing plate, 4 wash/eluate collection plates (2 ml), 4 eluate collection plates (0.35 ml), 4 blotting membranes, 2 sealing matspk
PAL-HT-PG-2D2 Optima PRO Screen, 2 Pressure sealing plate, 4 wash/eluate collection plates (2 ml), 4 eluate collection plates (0.35 ml), 4 blotting membranes, 2 sealing matspk
PAL-HT-PA-2D2 Optima PRO Screen, 2 Pressure sealing plate, 4 wash/eluate collection plates (2 ml), 4 eluate collection plates (0.35 ml), 4 blotting membranes, 2 sealing matspk
PAL-HT-PA-22 Optima PRO Screen, 2 Pressure sealing plate, 4 wash/eluate collection plates (2 ml), 4 eluate collection plates (0.35 ml), 4 blotting membranes, 2 sealing matspk
PAL-HT-NiADV-2D2 Optima PRO Screen, 2 Pressure sealing plate, 4 wash/eluate collection plates (2 ml), 4 eluate collection plates (0.35 ml), 4 blotting membranes, 2 sealing matspk
PAL-HT-NiADV-22 Optima PRO Screen, 2 Pressure sealing plate, 4 wash/eluate collection plates (2 ml), 4 eluate collection plates (0.35 ml), 4 blotting membranes, 2 sealing matspk
PAL-HT-HEP-2D2 Optima PRO Screen, 2 Pressure sealing plate, 4 wash/eluate collection plates (2 ml), 4 eluate collection plates (0.35 ml), 4 blotting membranes, 2 sealing matspk
PAL-HT-EMP-22 Optima PRO Screen, 2 Pressure sealing plate, 4 wash/eluate collection plates (2 ml), 4 eluate collection plates (0.35 ml), 4 blotting membranes, 2 sealing matspk
PRO96-50x50 De-salting platespk