제품안내  형광시약   Protein Labeling  CF™ Dyes

CF™594 Dye

비슷한 파장의 Alexa Fluor 594와 Texas Red에 비해 가장 밝으며, 형광이 오랫동안 지속되는 deep red 형광 dye입니다.

Biotium
    특징
  • 유사한 파장대의 형광 dye중 가장 밝으며, antibody cojugation yield가 높습니다.
  • 매우 형광이 오랫동안 지속됩니다.
  • 물에 잘 녹습니다.
    사양
  • Abs/Em Maxima: 593/614 nm
  • Extinction coefficient: 115,000
  • Molecular weight: ~730
  • Flow cytometry laser line: 532 or 594 nm
  • Microscopy laser line: 594 nm
  • Direct replacement for: Alexa Fluor® 594, Texas Red®, DyLight 594 and Cy™3
    적용
    Figure 1.Absorption and emission spectra of CF™594 conjugated to goat anti-mouse IgG in PBS.
    Figure 2.Relative fluorescence of CF™594- and Alexa Fluor® 594-goat anti-mouse conjugates as a function of number of dye per protein or degree of labeling (DOL). Fluorescence was measured at each dye’s emission maximum in PBS using 594 nm excitation.
    Figure 3.Jurkat cells were stained with intracellular CD3 followed by CF594 or AlexaFluor594 goat anti-mouse conjugates. Cells were continuously exposed using a mercury arc lamp microscope. Images were captured every 15 seconds for 5 min and fluorescence intensity of each frame was normalized to the first image

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