제품안내  형광시약   Protein Labeling  CF™ Dyes

CF™568 Dye

Alexa Fluor 568보다 더 밝고, 형광이 오랫동안 지속되는 red 형광 dye입니다.

Biotium
    특징
  • Alexa Fluor 568보다 더 밝으며, antibody cojugation yield가 높습니다.
  • 매우 형광이 오랫동안 지속됩니다.
  • 물에 잘 녹습니다.
    사양
  • Abs/Em Maxima: 562/583 nm
  • Extinction coefficient: 100,000
  • Molecular weight: 714
  • Flow cytometry laser line: 532 nm
  • Microscopy laser line: 568 nm
  • Direct replacement for: Alexa Fluor® 568, Lissamine rhodamine B, ROX
    적용
    Figure 1.Absorption and emission spectra of CF™568 conjugated to goat anti-mouse IgG in PBS.
    Figure 2.Jurkat cells were stained with intracellular CD3 or isotype control followed by goat anti-mouse IgG conjugates. Fluorescence was analyzed on a BD FACS Calibur in the FL2 channel. The bars represent the relative fluorescence of the geometric means of the population of cells.
    Figure 3.Jurkat cells were stained with intracellular biotin-CD3 followed by streptavidin CF568 or AlexaFluor568 conjugates. Cells were continuously exposed using a mercury arc lamp microscope. Images were captured every 15 seconds for 5 min and fluorescence intensity of each frame was normalized to the first image.

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