제품안내  항체,항원,킷트   BioAssay  Metabolism

Energy Metabolism Assay Kit

에너지대사에 관련된 세포내 물질을 측정하기 위해 제공되는 kit로, 96 well plate 및 cuvette내에서 시료를 측정할수 있습니다.

BioAssay Systems
Product용도SpecificationKit 구성품
QuantiChrom Free Amino Nitrogen Assay Kit 모든 식품, 음료 샘플에서 Free Amino Nitrogen 측정 (맥주, 맥아즙, 와인, 포도액 등) Procedure: 20 min
Linear detection range (5 µL sample): 0.2 to 10 mM for 10 min reaction
OD: 575nm
Reagent A: 18 mL
Reagent B: 600 μL
Standard: 500 μL (20 mM Glycine)
EnzyChrom™ Glutamine Assay Kit glutamine을 glutamate 로 가수분해하여 glutamine 농도를 측정하는 kit
colorimetric (565nm)
Procedure: 40 min. Detection limit: 23 μM. Shelf life: 3 months Assay Buffer: 15 mL
NAD Solution: 1 mL
Enzyme A: 120 μL
MTT Solution: 2 x 1.5 mL
Enzyme B: 220 μL
Stop Reagent: 25 mL
Standard: 400 μL 100 mM Glutamine
EnzyChrom™ Adipolysis Assay Kit 세포배양액에서 유리된 glycerol을 측정함으로서 adipolysis를 분석하기 위한 kit
Colorimetric (570nm)/fluorimetric (530/590nm) 정량
Procedure: 20 min
Detection limit: 0.92 μg/mL
Shelf life: 3 months
Assay Buffer: 24 mL
Enzyme Mix: 500 μL
ATP: 250 μL
Dye Reagent: 250 μL
Standard: 100 μL 100 mM Glycerol
EnzyLight™ ADP Assay Kit luminescence를 이용하여 시료내 ADP 농도를 빠르게 측정할 수 있는 kit Procedure: 20 min
Detection limit: 0.1 μM or 40 cells
Shelf life: 6 months
Assay Buffer: 10 mL
Substrate: 120 μL
Cosubstrate: 120 μL
ATP Enzyme: 120 μL
ADP Enzyme: 120 μL
Standard: 100 μL 3 mM ADP
EnzyLight™ ADP/ATP Ratio Assay Kit luminescence를 이용하여 시료내 ADP/ATP ratio를 정량할 수 있는 kit Procedure: 20 min
Shelf life: 6 months
Assay Buffer: 10 mL
Substrate: 120 μL
Cosubstrate 120 μL
ATP Enzyme: 120 μL
ADP Enzyme: 120 μL
EnzyLight™ ATP Assay Kit luminescence를 이용하여 세포내 ATP농도를 빠르게 정량할 수 있는 kit Procedure: 10 min
Detection limit: 0.1 μM or 40 cells
Shelf life: 6 months
Assay Buffer: 10 mL
Substrate: 120 μL
ATP Enzyme: 120 μL
Standard: 100 μL 3 mM ATP
QuantiChrom™ DNA Assay Kit DNA를 Fluorimetric (340/450nm)으로 측정 Procedure: 5 min
Detection limit: 100 ng/mL
Shelf life: 12 months
Reagent: 50 mL
Standard: 1 mL 10 μg/mL calf thymus DNA
QuantiChrom™ Ethanol Assay Kit ethanol을 Chemical colorimetric (580nm)으로 측정 Procedure: 10 min
Detection limit: 0.04%
Shelf life: 12 months
Reagent A: 50 mL
Reagent B: 50 mL
10% TCA: 50 mL
Standard: 2 mL 10% (v/v) ethanol
EnzyChrom™ Ethanol Assay Kit ethanol을 enzymatic (565nm)으로 정량 Procedure: 5 min
Detection limit: 0.0003%
Shelf life: 6 months
Assay Buffer: 10 mL
NAD Solution: 1 mL
PMS Solution: 1.5 mL
MTT Solution: 1.5 mL
Enzyme: 120 μL
Standard: 1.5 mL 1% (v/v) ethanol
QuantiChrom™ Glucose Assay Kit glucose를 chemical colorimetric (630nm) 으로 정량 Procedure: 10 min
Detection limit: 0.7 mg/dL (39 μM)
Shelf life: 12 months
Tests (96-well): 100
Reagent: 50ml
Standard : 1 mL 300 mg/dL
QuantiChrom™ Glucose Assay Kit glucose를 chemical colorimetric (630nm) 으로 정량 Procedure: 10 min
Detection limit: 0.7 mg/dL (39 μM)
Shelf life: 12 months
Tests (96-well): 200
Reagent: 100ml
Standard : 1 mL 300 mg/dL
EnzyChrom™ Glucose Assay Kit glucose를 colorimetric (570nm)이나 fluorometric (530/590nm)으로 정량 Procedure: 40 min
Detection limit: 5 μM
Shelf life: 3 months
Assay Buffer: 10 mL
Enzyme Mix: 120 μL
Dye Reagent: 120 μL
Standard: 1 mL 300 mg/dL Glucose
QuantiChrom™ α-Glucosidase Assay Kit α-glucosidase의 활성을 colorimetric (405nm)으로 정량 Procedure: 20 min
Detection limit: 2 U/L
Shelf life: 12 months
Assay Buffer: 12 mL (pH 7.0)
a-NPG Substrate: 1 mL
Calibrator: 10 mL (equivalent to 250 U/L)
QuantiChrom™ β-Glucosidase Assay Kit β-glucosidase의 활성을 colorimetric (405nm)으로 정량 Procedure: 20 min
Detection limit: 2 U/L
Shelf life: 12 months
Assay Buffer: 12 mL (pH 7.0)
b-NPG Substrate: 1 mL
Calibrator: 10 mL (equivalent to 250 U/L)
EnzyChrom™ Glycogen Assay Kit glycogen의 농도를 colorimetric (585nm)으로 정량 Procedure: 20 min
Detection limit: 0.9 μg/mL
Shelf life: 6 months
Assay Buffer: 20 mL
Enzyme A: 120 μL
Enzyme B: 2 x 120 μL
Standard: 50 μL 50 mg/mL Standard
Detection Reagent: 20 mL
EnzyChrom™ HDL and LDL/VLDL Assay Kit 혈청의 HDL과 LDL/HDL 콜레스테롤 농도를 enzymatic (340nm)으로 정량 Procedure: 60 min
Detection limit: 5 mg/dL
Shelf life: 6 months
PBS: 1.5 mL
Precipitation Reagent: 1.5 mL
Assay Buffer: 12 mL
Enzyme Mix: 120 uL
NAD Solution: 2 mL
Standard: 1 mL 300mg/dL cholesterol
EnzyChrom™ L-Lactate Assay Kit L-Lactic acid의 농도를 enzymatic (565nm)으로 정량 Procedure: 20 min
Detection limit: 0.05 mM
Shelf life: 6 months
Assay Buffer: 6 mL
NAD Solution: 1 mL
PMS Solution: 1.5 mL
MTT Solution: 1.5 mL
Enzyme: 120 μL L-LDH
Standard: 1.0 mL 20 mM L-Lactate
EnzyChrom™ D-Lactate Assay Kit D-Lactic acid의 농도를 enzymatic (565nm)으로 정량 Procedure: 20 min
Detection limit: 0.05 mM
Shelf life: 6 months
Assay Buffer: 6 mL
NAD Solution: 1 mL
PMS Solution: 1.5 mL
MTT Solution: 1.5 mL
Enzyme: 120 μL D-LDH
Standard: 1.0 mL 20 mM D-lactate
EnzyChrom™ NAD/NADH Assay Kit NAD/NADH의 농도를 enzymatic (565nm)으로 정량 Procedure: 15 min
Detection limit: 0.2 μM
Shelf life: 6 months
Assay Buffer: 10 mL
Ethanol (1 vol%): 1.5 mL
PMS Solution: 1.5 mL
MTT Solution: 1.5 mL
Enzyme: 120 μL
NAD Standard: 0.5 mL 1 mM
NAD/NADH Extraction Buffers: each 12 mL
EnzyChrom™ NADP/NADPH Assay Kit NADP/NADPH의 농도를 enzymatic (565nm)으로 정량 Procedure: 30 min
Detection limit: 0.1 μM
Shelf life: 6 months
Assay Buffer: 10 mL
Glucose (1 M): 1.5 mL
PMS Solution: 1.5 mL
MTT Solution: 1.5 mL
Enzyme (GDH): 120 μL
NADP Standard: 0.5 mL 1 mM
NADP/NADPH Extraction Buffers: each 12 mL
QuantiChrom™ Protein Assay Kit 개선된 Coomassie Blue G 방법을 이용하여 단백질 농도를 595nm에서 정량 Procedure: 2 min
Detection limit: 60 μg/mL
Shelf life: 12 months
Reagent: 20 mL 5 x concentrate
Protein standard: 1 mL 1.0 mg/mL BSA
Saccharide Removal Kit Ethanol Assay에 방해가 되는 샘플 속의 glucose나 sucrose와 같은 당을 제거해 주는 kit Shelf life: 12 months Reagent A: 20 mL
Reagent B: 100 mL
Reagent C: 20 mL
EnzyChrom™ Starch Assay Kit a-amylase/amyloglucosidase hydrolysis 시약을 이용하여 빠르게 당 속의 전분을 분해하여 전분의 농도를 colorimetric (585nm)으로 정량 Procedure: 20 min
Detection limit: 0.6 μg/mL
Shelf life: 6 months

Assay Buffer: 20 mL
Enzyme A: 120 μL
Enzyme B: 120 μL
Standard: 50 μL 50 mg/mL starch
Detection Reagent: 20 mL

EnzyChrom™ Acetaldehyde Assay Kit biological and food/beverage samples에서 Acetaldehyde 정량.
565 nm에서 컬러 측정
Detection Limit: 4 µM

Assay Buffer: 10 mL
NAD/MTT Solution: 1 mL
Enzyme A: 120 μL
Enzyme B: 120 μL
Standard: 100 μL

QuantiFluo™ Cholesterol Uptake Assay Kit 유착 세포에 의한 콜레스테롤 섭취, 콜레스테롤 섭취 억제제 스크리닝, 콜레스테롤 흡수에 대한 약물의 효과 평가. Method: FL485/535nm

Assay Reagent: 12 mL
Fluorescent Tracer: 250 μL
Positive Control (2.5 mM): 20 μL


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