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Optima PRO Screens & De-salting

Optima PRO Screen پ ܹ ϱ ̿˴ϴ. ִ 96 ٸ ̹ resin coating plate ֽϴ. (resin ÿ ° ֽϴ.)

Protein Ark

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  • Filtration purification ѹ ִ 96 well screen plate Դϴ.
  • well resin óǾ ѹ ٷ ܹ ֽϴ.
  • Secreted protein ó ٷ cell culture εմϴ.
  • Ƿ ɺи ͸ ó ʿϴ.
  • иϰ ϴ ܹ Resin ֽϴ.

Optima Pro Empty Plate Optima PRO-D Ni Advance plate Optima PRO-D Protein A plate Optima PRO-D Protein G plate Optima PRO-D Q-IEX plate Optima PRO-D S-IEX plate
Resin Resin dependent Fastback Ni Advance Fastback Protein A Fastback Protein G Fastback Q-IEX Fastback S-IEX
Specificity Resin dependent His - tag IgG, Fc resion IgG, Fc resion Negatively charged proteins Positively charged proteins
Matrix Resin dependent 6% cross-linked Agarose Sepharose Sepharose 6% cross-linked Agarose 6% cross-linked Agarose
Coupled ligand Resin dependent Ni chelating ligand 3.5 mg
Protein A /ml
2 mg
Protein G /ml
Quaternary ammonium Sulphonic acid propyl
Binding capacity Resin dependent 80 mg/ml 30 mg/ml 20 mg/ml 50 - 70 mg/ml 50 - 70 mg/ml
Bead size Resin dependent 90um 60 - 165 um 45 - 165 um 90 - 100 um 90 - 100 um
DTT stability (24 hours) Resin dependent 20mM - - - -
EDTA stability (24 hours) Resin dependent 20mM - - - -
Buffer compatibility Common aqueous buffers from pH 2-12 Common aqueous buffers from pH 4-9 Common aqueous buffers from pH 2.5-10 Common aqueous buffers from pH 2-9 Common aqueous buffers from pH 2-12 Common aqueous buffers from pH 2-12
Resin volume per well 50ul - 100ul 50ul
Diatomaceous Earth No Yes
Max recommended sample volume 1ml
plastic material Polypropylene
Membrane material PTFE
Recommended vacuum pressure -0.6 bar (max. vacuum -0.8 bar)
Recommended centrifuge speed 2,000 - 3,000 x g
Recommended shaker speed (2mm orbit) 800 rpm
Maximum shaker speed (2mm orbit) 1,200 rpm
Storage temperature Ambient 2 - 8






Sample type Optima PRO-D
(pre-packed with resin and Diatomaceous Earth)
Intact Mammalian cells (HEK/CHO) Unclarified sample
(Up to 15 x 10^6 cells/ml)
Intact Insect cells (Sf9/Hi5) Unclarified sample
(Up to 15 x 10^6 cells/ml)
Bacterial cell lysate (E.coil) Pre-clarification of sample
(5,000 x g for 15 mins in 2ml deep well block)
Mammalian cell lysate (HEK/CHO) Pre-clarification of sample
(5,000 x g for 15 mins in 2ml deep well block)
Insect cell lysate (Sf9/Hi5) Pre-clarification of sample
(5,000 x g for 15 mins in 2ml deep well block)



  • ٷ ѹ Żϰų ϴµ ̿ϴ plate Դϴ.
  • De-salting 8, Buffer exchange 12 ҿ˴ϴ.









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