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 Á¦Ç°¾È³»  ºÐÀÚ»ý¹°ÇР  PCR Amplification  Direct PCR

Direct PCR Technology

What is the Direct PCR?
Direct PCRÀº DNA ÃßÃâ°úÁ¤¾øÀÌ ¼Ò·®ÀÇ »ùÇ÷Π¹Ù·Î PCRÀ» ÇÏ´Â ±â¼úÀÔ´Ï´Ù. PCRÀ» ÇϱâÀ§ÇØ ¹Ýµå½Ã °ÅÃÄ¾ß Çß´ø DNA ÃßÃâ°úÁ¤Àº, °¢ »ùÇÿ¡ ¸Â´Â DNA kitÀ» ±¸ÀÔÇÏ¿© DNA ÃßÃâ½ÇÇèÀ» ÇØ¾ß Çϰí, ¿©·¯´Ü°è¸¦ °ÅÄ¡´Â µ¿¾È »ùÇÃÀÇ ¿À¿°ÀÌ ¹ß»ýÇÒ ¼ö ÀÖÀ¸¸ç, »ùÇÃÀÇ ¾çÀÌ ÀûÀ»¶§´Â »ùÇà ¼Õ½ÇÀÌ ¸¹Àº ¾î·Á¿òÀÌ ÀÖ¾ú½À´Ï´Ù. Direct PCR ¹æ¹ýÀº ÀÌ·¯ÇÑ ´Ü°è¸¦ »ý·«ÇÏ¿©, ¹Ù·Î »ùÇ÷κÎÅÍ PCRÀ» ÁøÇàÇϹǷÎ, ½Ã°£°ú ºñ¿ëÀÌ Àý¾àµÇ°í, ¿À¿°°ú ¼Õ½ÇÀÇ À§ÇèÀ¸·ÎºÎÅÍ ¾ÈÀüÇÕ´Ï´Ù. ÀÌ ±â¼úÀº blood, ¿À·¡µÈ Ç÷¾×, saliva, bacteria, mouse tail ¹× tissue, cultured cell, plantµî, ´Ù¾çÇÑ »ùÇÿ¡ »ç¿ëµÉ ¼ö ÀÖ½À´Ï´Ù.

°í¸¶¿¡¼­´Â ¿©·¯°¡Áö ŸÀÔÀÇ Direct PCR Á¦Ç°À» Á¦°øÇÕ´Ï´Ù.
¿©·¯ ºê·£µåÀÇ ´Ù¾çÇÑ Taq polymerase¿Í ÇÔ²² ȣȯÇÏ¿© »ç¿ëÇÒ ¼ö ÀÖ´Â Direct PCR Buffer »Ó ¾Æ´Ï¶ó, °í¸¶ÀÇ Taq°ú ÇÔ²² È¥ÇÕµÈ Direct PCR MasterMix ŸÀÔÀÇ Á¦Ç°µµ Á¦°øÇÔÀ¸·Î½á, Primer¿Í template¸¸ ÁغñÇÏ¿© ¹Ù·Î ½ÇÇèÇÏ½Ç ¼ö ÀÖµµ·Ï Æí¸®ÇÑ ¿©°ÇÀ» ¸¶·ÃÇØ µå¸³´Ï´Ù. ¶ÇÇÑ, mouse tail À̳ª plantµîÀÇ »ùÇÿ¡ ÃÖÀûÈ­µÈ kitµµ ½Ã°£°ú ½ÇÇè°úÁ¤À» °³¼±½ÃÄÑ µå¸³´Ï´Ù.

ÀÌÁ¦ Direct PCR·Î ¼Õ½±°Ô Genotyping Çϼ¼¿ä!
KOMA Direct PCR Advantages
Features EzWay Direct PCR Buffer Conventional PCR Method
No DNA extraction required ¡á ¡à
Save Time and Cost ¡á ¡à
Less Risk of DNA Contamination ¡á ¡à
No Loss of Samples ¡á ¡à
Compatibility with any other Taq Enzymes ¡á ¡à
Format BUFFER
Product Direct PCR Buffer Direct PCR Buffer Set
Cat. No K0568001 K0568002
±×¸²
Ư¡ Direct PCRÀ» À§ÇÑ ¼ººÐÀÌ Æ÷ÇÔµÈ Taq reaction buffer·Î, Taq°ú ÇÔ²² »ç¿ëµÇ´Â ÀÏ¹Ý Reaction buffer ´ë½Å ÀÌ ¹öÆÛ¸¦ ±³Ã¼»ç¿ëÇÔÀ¸·Î½á, Direct PCRÀÌ °¡´ÉÇØ Áý´Ï´Ù. Blood °°ÀÌ À¯¿¬ÇÑ »ùÇÿ¡ ÀûÇÕÇÕ´Ï´Ù. Direct PCR Buffer¿Í ÇÔ²², »ùÇà 󸮿¡ ÇÊ¿äÇÑ Lysis buffer ¹× high GC content¿¡ À¯¿ëÇÑ Magic buffer°¡ Á¦°øµÇ´Â buffer set ÀÔ´Ï´Ù. Á¶Á÷»ùÇð°Àº, lysis°¡ ÇÊ¿äÇÑ »ùÇÿ¡ ÀûÇÕÇÕ´Ï´Ù.
Component Direct PCR Buffer Direct PCR Buffer
Direct Lysis Buffer I
Direct Lysis Buffer II
Magic Buffer
Sample Á¶Á÷ÀÌ ¿¬ÇÑ »ùÇÃ:
Blood (whole blood, dried blood, blood on paper), Buccal swab, Saliva, Sputum, Urine¡¦
Á¶Á÷ÀÌ ´Ü´ÜÇÑ »ùÇÃ:
Mouse tail, Animal tissue, Plant, Cultured cell, Hair root, sperm,¡¦
»ç¿ë¹ý
  1. Blood¸¦ ÁغñÇÑ´Ù.
  2. 1ul¸¦ »ç¿ëÇÏ¿© ¹Ù·Î PCR.
  1. »ùÇÃÀ» ÁغñÇÑ´Ù.
  2. »ùÇÿ¡µû¶ó ÀûÇÕÇÑ Áغñ¹æ¹ýÀ» µû¸¥´Ù.
  3. 1ul¸¦ »ç¿ëÇÏ¿© ¹Ù·Î PCR.
»ç¿ëȽ¼ö 125 PCR rx (20ul each)
50 PCR rx (50ul each)
125 PCR rx (20ul each)
50 PCR rx (50ul each)
Format MASTERMIX
Product Direct MasterMix Direct Hot Taq PCR MasterMix Direct Hot Universal qMasterMix
Cat. No K0568010 K0568210 K0569020
±×¸²
Ư¡ Direct PCR Buffer°¡ ÀÌ¹Ì Taq, dNTP, MgCl2, dyeµîÀÇ ¿ä¼Ò¿Í ÇÔ²² MasterMix ÇüÅ·ΠÁ¦°øµË´Ï´Ù. °í°´ÀÌ Mix¸¦ ¸¸µé ÇÊ¿ä¾øÀÌ, template¿Í PCR¸¸ ÁغñÇÏ¿© ¼¯¾î »ç¿ëÇÕ´Ï´Ù. Direct PCR Buffer°¡ ÀÌ¹Ì Hot Taq, dNTP, MgCl2, dyeµîÀÇ ¿ä¼Ò¿Í ÇÔ²² MasterMix ÇüÅ·ΠÁ¦°øµË´Ï´Ù. °í°´ÀÌ Mix¸¦ ¸¸µé ÇÊ¿ä¾øÀÌ, template¿Í PCR¸¸ ÁغñÇÏ¿© ¼¯¾î »ç¿ëÇÕ´Ï´Ù. Hot start È¿°ú¸¦ ¾ò±âÀ§ÇØ ÀûÇÕÇÕ´Ï´Ù. Direct PCR Buffer°¡ ÀÌ¹Ì Hot Taq, dNTP, MgCl2, dyeµîÀÇ ¿ä¼Ò¿Í ÇÔ²² MasterMix ÇüÅ·ΠÁ¦°øµË´Ï´Ù. Hot TaqÀ» ÀÌ¿ëÇÑ real time PCR¿ë MasterMixÀÔ´Ï´Ù.
Component Direct Taq PCR Master Mix
Magic Buffer
Direct Hot Taq PCR Master Mix
Magic Buffer
Direct Hot Universal qMasterMix
Magic Buffer
Sample ¸ðµç»ùÇÃ
(´Ü, Lysis buffer´Â °³º° ±¸¸ÅÇØ¾ß ÇÔ)
¸ðµç»ùÇÃ
(´Ü, Lysis buffer´Â °³º° ±¸¸ÅÇØ¾ß ÇÔ)
¸ðµç»ùÇÃ
(´Ü, Lysis buffer´Â °³º° ±¸¸ÅÇØ¾ß ÇÔ)
»ç¿ë¹ý
  1. »ùÇÃÀ» ÁغñÇÑ´Ù.
  2. »ùÇÃÀÌ Blood°°ÀÌ À¯¿¬ÇÑ »ùÇÃÀÌ¸é ¹Ù·Î 1ul¸¦ ÁغñÇϰí, µÎ²¨¿î Á¶Á÷ »ùÇÃÀ̸é, lysis¸¦ °ÅÄ£´Ù.
  3. 1ul¸¦ MasterMix¿¡ ³Ö¾î ¹Ù·Î PCR.
  1. »ùÇÃÀ» ÁغñÇÑ´Ù.
  2. »ùÇÃÀÌ Blood°°ÀÌ À¯¿¬ÇÑ »ùÇÃÀÌ¸é ¹Ù·Î 1ul¸¦ ÁغñÇϰí, µÎ²¨¿î Á¶Á÷ »ùÇÃÀ̸é, lysis¸¦ °ÅÄ£´Ù.
  3. 1ul¸¦ MasterMix¿¡ ³Ö¾î ¹Ù·Î PCR.
  1. »ùÇÃÀ» ÁغñÇÑ´Ù.
  2. »ùÇÃÀÌ Blood°°ÀÌ À¯¿¬ÇÑ »ùÇÃÀÌ¸é ¹Ù·Î 1ul¸¦ ÁغñÇϰí, µÎ²¨¿î Á¶Á÷ »ùÇÃÀ̸é, lysis¸¦ °ÅÄ£´Ù.
  3. 1ul¸¦ MasterMix¿¡ ³Ö¾î ¹Ù·Î PCR.
»ç¿ëȽ¼ö 100 PCR rx (10ul each)
40 PCR rx (25ul each)
100 PCR rx (10ul each)
40 PCR rx (25ul each)
100 PCR rx (10ul each)
40 PCR rx (25ul each)
Format KIT
Product EzWay¢â Mouse Tail Direct PCR Kit EzWay¢â Plant Direct PCR Kit
Cat. No K0568600 K0568700
±×¸²
Ư¡ Direct PCR Buffer¿Í Taq MasterMix·Î, Mouse tail Á¶Á÷¿¡ Á»´õ ÃÖÀûÈ­µÈ »óÅ·ΠÁ¦°øµË´Ï´Ù. Direct PCR Buffer¿Í Taq MasterMix·Î, plant Á¶Á÷¿¡ Á»´õ ÃÖÀûÈ­µÈ »óÅ·ΠÁ¦°øµË´Ï´Ù.
Component Direct Taq PCR Master Mix
Mouse Tail Lysis buffer
Magic Buffer
Direct Taq PCR Master Mix
Plant Lysis buffer
Magic Buffer
Sample Mouse tail, ear Plant leaves
»ç¿ë¹ý
  1. TailÀ» ÀÚ¸¥´Ù.
  2. Lysis buffer¿¡ ³Ö°í 60¡ÆC 10ºÐ ¹ÝÀÀ
  3. Vortex and spin down.
  4. 1ul »óÃþ¾×À» MasterMix¿¡ ³Ö¾î ¹Ù·Î PCR.
  1. LeafÀ» ÀÚ¸¥´Ù.
  2. Lysis buffer¿¡ ³Ö°í 85¡ÆC 10ºÐ ¹ÝÀÀ
  3. Vortex and spin down.
  4. 1ul »óÃþ¾×À» MasterMix¿¡ ³Ö¾î ¹Ù·Î PCR.
»ç¿ëȽ¼ö 100 PCR rx (10ul each)
40 PCR rx (25ul each)
100 PCR rx (10ul each)
40 PCR rx (25ul each)